The Mathura police is part of the Uttar Pradesh police & responsible for maintaining Law and Order enforcement in Mathura District & Mathura City. Mathura district comes under the Agra Police Zone and Agra Police Range, Agra Zone is headed by an additional director general (ADG)-ranked Indian Police Service (IPS) officer, and the Agra Range is headed by a deputy inspector general (DIG)-ranked IPS officer.

District Police of Mathura is headed by the Senior Superintendent of Police (SSP).  He is assisted by Superintendents of Police (SPs) and additional Superintendent of Police (Addl. SPs) (City, Rural Area, Traffic, Security, and Crime). SP City looks after the law and order of Police Stations in the Urban Areas. SP Rural looks after the law and order of Police Stations in Rural Areas. SP Traffic takes care of the traffic in the whole of the district and SP Crime looks after the crime department

The district is further divided into numerous police circles, each headed by a Circle Officer in the rank of Deputy Superintendent of Police. SP (Traffic) and SP (Crime) are assisted by one Circle Officer in the rank of Deputy Superintendent of Police. 

Police Stations in Mathura come under the circle of officers (CO). Each Circle is headed by the Deputy superintendent of police. The Circles in Mathura are CO City, Sadar, Refinery, Chhath, Goverdham, Mahavam & Manth. Mathura District has 22 Law and order Police Stations.